For applying the animal alternative test data on chemical assessment, the new information for alternative test strategies, nano-substance registration, and the recent development of alternative test methods were continuously collected and analyzed. Furthermore, the list of alternative test methods was updated, and 2 guidance documents for how to use alternative tests were established (“Guidance on Alternatives of Acute Dermal Irritation and Corrosion” and “Guidance on Alternatives of Eye Irritation”). To strengthen the application of alternative methods on toxicological and eco-toxicological information required by chemical substances registration, quantitative structure-activity relationship (QSAR) models were used to predict the health and environmental hazards of 15 substances (22 substances in total). The choose QSAR models showed good performances on genotoxicity. Danish QSAR Database and the group contribution method in Toxicity estimation software tool (T.E.S.T.) also provided well predictions on the classification of acute fish toxicity. This year, we also evaluated the acute fish toxicity of 5 substances (25 substances in total) using fish embryo acute toxicity Test (FET). By combining the data of QSAR (T.E.S.T. Group contribution method and ECOSAR) and FET, we successfully optimized the integrated approaches to testing and assessment (IATA). Our results showed that the accuracy of IATA could reach 88%. In the other hand, we also predicted the physical and chemical properties and environmental fate of 10 organotin compounds by 2 different QSAR model. The classification accuracy for class 1 toxic chemical substances could reach 88%. For avoiding unnecessary animal tests, the bioconcentration factor (BCF) of 106 substances which should be completed standard registration before 2024 were predicted by 11 kinds of QSAR and the accuracy of prediction reached 95.8%.

Replacement, Reduction and Refinement (3Rs), Alternative test methods, Integrated Approaches to Testing and Assessment (IATA)